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Diagnostic tests for fertility treatment (IVF) and early detection of pregnancy disorders

LH/hCG receptor ELISA: Diagnostic tests for fertility treatment (IVF) and early detection of pregnancy disorders

Origin Biomarkers has developed three novel diagnostics. These tests measure the blood concentration of the soluble (circulating) form of LHCGR and its complex with hormones LH and hCG by ELISA.

Test 1: sLHCGR alone
Test 2: LH-sLHCGR (Hormone-receptor complex)
Test 3: hCG-sLHCGR (Hormone-receptor complex)
Under Development & Validation: TSH-LHCGR

Background of the tests

Under the direction of gonadotropin releasing hormone (GNRH) derived from the hypothalamus (brain), the pituitary secretes luteinizing hormone (LH) which stimulates the ovary and testis leading to the production of estrogen and androgens (testosterone) in women and men respectively. However, in a feed-back mechanism, the hypothalamic pituitary gonadal (HPG) axis is negatively regulated by gonadal steroids. LH is a homologue of pregnancy hormone human chorionic gonadotropin (hCG). In addition to placenta, hCG is also secreted from pituitary in women at menopause.

LH and hCG both bind to a common receptor termed LHCGR which is expressed in numerous tissues including adrenal glands, brain, skin, retina etc as well as the reproductive organs. The non-gonadal expression of the LHCGR has been shown to be necessary for local steroid production. Diabetes, thyroid diseases, renal dysfunctions, advanced age, inflammatory conditions, stress etc support the release of soluble LHCGR from the inflamed tissues into the circulation.

One in eight women & men suffer infertility

Every year 15 million babies are born premature (WHO)

Discovery

Secretion of sLHCGR from tissues followed by detection of sLHCGR in blood and follicular fluid.

Technology

Based on unique biomarkers for EARLY detection of preeclampsia and other pregnancy diseases/infertility

Applications

IVF / Fertility Treatments
Oocyte yield, Early Pregnancy loss, Premature birth, Low/high birth weight Reduce multiple births without compromising success rate.
Pregnancy
Premature Birth, Miscarriage, Down’s Syndrome, Preeclampsia
Other
Testing for early development of cognitive failures and Alzheimer’s

Established October 2008

History in innovating in medical diagnostic tests

Advanced medical diagnostic tests

Unique biomarker testing for early detection for pregnancy related conditions

Intellectual Property

Patents granted (USA & EPO) for diagnostic and therapeutic applications of soluble LHCGR protein.

Our Publications on LH/hCG receptor

Banerjee S, Smallwood A, Chambers AE, Papageorghiou A, Loosfelt H, Spencer K, Campbell S, Nicolaides K: A link between high serum levels of human chorionic gonadotrophin and chorionic expression of its mature functional receptor (LHCGR) in Down's syndrome pregnancies. Reprod Biol Endocrinol2005, 3:25
Abstract | HTML | PDF

Chambers AE, Stanley PF, Randeva H, Banerjee S. Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants. Reprod Biol Endocrinol. 2011 May 15;9:64. doi: 10.1186/1477-7827-9-64.
Abstract | HTML | PDF

Chambers AE, Nayini KP, Mills WE, Lockwood GM, Banerjee S. Circulating LH/hCG receptor (LHCGR) may identify pre-treatment IVF patients at risk of OHSS and poor implantation. Reprod Biol Endocrinol. 2011 Dec 23;9:161. doi: 10.1186/1477-7827-9-161.
Abstract | HTML | PDF

Chambers AE, Griffin C, Naif SA, Mills I, Mills WE, Syngelaki A, Nicolaides KH, Banerjee S. Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, Down's syndrome, preterm delivery and preeclampsia. Reprod Biol Endocrinol. 2012 Dec 17;10:113. doi: 10.1186/1477-7827-10-113.
Abstract | HTML | PDF

Chambers AE, Mills WE, Mercadé I, Crovetto F, Crispi F, Bodi LR, Pugia M, Mira A, Lasalvia L, Banerjee S, Casals E, Gratacos E. The utility of circulating LHCGR as a predictor of Down's syndrome in early pregnancy. BMC Pregnancy Childbirth. 2014 Jun 6;14:197. doi: 10.1186/1471-2393-14-197.
Abstract | HTML | PDF

Crovetto F, Figueras F, Crispi F, Triunfo S, Pugia M, Lasalvia L, Chambers AE, Mills WE, Banerjee S, Mercadé I, Casals E, Mira A, Rodriguez-Revenga Bodi L, Gratacós E.
Forms of Circulating Luteinizing Hormone Human Chorionic Gonadotropin Receptor for the Prediction of Early and Late Preeclampsia in the First Trimester of Pregnancy.
Fetal Diagn Ther. 2015 Feb 11. [Epub ahead of print]
Abstract